This article is rated PG, meaning it contains content that may be inappropriate for users the age of 8 and under.
The story begins with Tom, Jerry and Tuffy find themselves involved with Star-Lord and some alien criminals on a quest to prevent Ronan the Accuser, a mad Kree, from abusing the Power-Stone, one of the Infinity Stones, to start a war with Earth.
- Tom Cat - A light-blue cat, who is Star-Lord's pet cat, Jerry's rival and friend and Tuffy's friend, Toodles' husband and Tin, Pan and Alley's ex-boss.
- Jerry Mouse - A brown-orange mouse, who is Star-Lord's pet mouse, Tom's rival and friend and Tuffy's uncle and Cherie's husband.
- Tuffy Mouse - A young baby light-grey, diaper-wearing mouse, who is Star-Lord's pet baby mouse, Jerry and Cherie's nephew and Tom's friend.
- Star-Lord -
- Gamora -
- Drax the Destroyer -
- Groot -
- Rocket Raccoon -
- Ronan the Accuser -
- Yondu Udonta
- Nebula -
- Toodles Galore -
- Cherie Mouse -
- Thanos -
- Tin, Pan and Alley -
- Richard Kind as Tom Cat
- Stephanie Nadolny as Jerry Mouse
- Kath Soucie as Tuffy Mouse, Gamora
- Chris Pratt as Peter Quill / Star-Lord
- David Sobolov as Drax the Destroyer
- Brad Garrett as Groot
- Frank Welker as Baby Groot
- Trevor Devall as Rocket Raccoon
- James Arnold Taylor as Ronan the Accuser
- John Barrowman as Yondu Udonta
- Grey DeLisle as Nebula, Toodles Galore
- Tara Strong as Cherie Mouse
- Jim Cummings as Thanos
- Greg Ellis as Tin Cat
- Jess Harnell as Pan Cat
- Richard McGonagle as Alley Cat
- Rated PG (Parental Guidance) with moderate fantasy action violence, mild threat, injury detail, very mild language.
- Suggested Running Times: 122 Minutes (NTSC), 117 Minutes (PAL).
- Kath Soucie voices Gamora with the voice she did for Sally Acorn on Sonic The Hedgehog.
- James Arnold Taylor voices Ronan with the voice he did for The Fallen on Transformers: Revenge of the Fallen (game).