Tom and Jerry: Fifty Shades Of Mouse is a American erotic romantic drama film, produced by Warner Bros. Pictures (WarnerMedia Byline), Warner Bros. Animation, Universal Pictures, Metro-Goldwyn-Mayer Pictures, Perfect World Pictures and Trigger Street Productions based on (Fifty Shades of Grey 2015-film, Fifty Shades Darker 2017-film and Fifty Shades Freed 2018-film).
- Jerry Mouse - A brown-orange mouse, who is Cherie's husband, Tuffy and Lucy's uncle, Grandpa and Mrs. Mouse's son, Geraldine's brother, Olive's friend, Muscles' cousin, Uncle Harry and Pecos grandnephew and Tom's rival.
- Cherie Mouse -
- Tuffy Mouse -
- Lucy Mouse -
- Grandpa Mouse -
- Geraldine Mouse -
- Olive Mouse -
- Mrs. Mouse -
- Muscles Mouse -
- Uncle Harry -
- Uncle Pecos -
- Tom Cat -
- Toodles Galore -
- Thomasina Cat -
- Anastasia "Ana" Steele - A 21-year-old English Literature student attending Washington State University, who is Christian's wife, Tom, Jerry and Tuffy's owner and friend.
- Christian Grey - A 27-year-old billionaire, entrepreneur, and CEO of Grey Enterprises Holdings Inc, who is Anastasia's husband, Tom, Jerry and Tuffy's owner and friend.
- Katherine "Kate" Kavanagh - Anastasia's best friend/roommate who begins a relationship with Christian's older brother, Elliot Grey.
- Carla Wilks - Anastasia's mother and Mrs. Mouse's owner and friend.
- Dr. Grace Trevelyan-Grey - Christian Grey's adoptive mother.
- Elliot Grey - Christian's adopted older brother.
- José Rodriguez - One of Anastasia's close friends.
- Jason Taylor - Christian's bodyguard and head of his security.
- Ray Steele - Anastasia's father.
- Carrick Grey - Christian's adoptive father.
- Bob Adams - Anastasia's stepfather.
- Paul Clayton - The brother of the owner of Clayton's Hardware Store.
- Martina -
- Andrea - Christian's assistant.
- Jennifer Hale as Carla Wilks
- Rated PG (Parental Guidance) with mild sex, violence, threat, language.
- Suggested Running Times: 130 Minutes (NTSC), 125 Minutes (PAL).