After the events of Spider-Man: Far From Home, Peter Parker was proved innocent after the late Mysterio showed his identity to the world and blamed him for the Europe chaos. Peter is taken to a bunker in Canada alongside Aunt May. In the bunker, Peter meets Madame Web, an elderly and blind woman that can see the future, and Cindy Moon, a girl with spider-powers used in an illegal experiment. Meanwhile, Mysterio was revealed to fake his death, and he assembles a team of villains to kill Spider-Man: The Sinister Six.
When the Sinister Six escalate to a world threat, Prowler, a super-hero who took Spider-Man´s place, sends a desperate call to Spider-Man, to help him defeat the villains.
- Tom Holland as Peter Parker/Spider-Man.
- Tiffany Espensen as Cindy Moon/Silk.
- Meryl Streep as Cassandra Webb/Madame Webb.
- Marisa Tomei as May Parker.
- Zendaya as Michelle Jones.
- Jacob Batalon as Ned Leeds.
- Jake Gyllenhaal as Quentin Beck/Mysterio.
- Michael Keaton as Adrian Toomes/Vulture.
- Jason Momoa as Sergei Kravinoff/Kraven.
- Michael Mando as Mac Gargan/Scorpion.
- Bokeem Woodbine as Herman Schultz/Shocker.