The film is produced by Allspark Pictures and DHX Media, with animation created once again using Toon Boom Harmony, and is scheduled to be released theatrically on October 2, 2020 in the United States by Lionsgate. It also marks the 10th Anniversary of My Little Pony: Friendship is Magic.
A giant whirlwind sends Onyx Star, Adonis, the weasels, Nike, Discord and the ostriches to outer space and they need to get back home to Equestria. Along the way, they meet new friends who were also stuck in space years ago, space animals, and evil alien ponies who wanted to capture Onyx and his friends and destroy the Earth with a reliquary-cannon after Groff, Maxil, Zio and their minions sold all of their souls for it.
- Tara Strong as Twilight Sparkle, a leader of Mane 6.
- Ashleight Ball as Rainbow Dash, a member of Mane 6, and Applejack, a member of the Mane 6.
- Andrea Libman as Pinkie Pie, a member of Mane 6, and Fluttershy, a member of the Mane 6.
- Tabitha St. Germain as Rarity, a member of the Mane 6.
- Cathy Weseluck as Spike, a member of the Mane 6 and Twilight's No. 1 Assistant.
- Emily Blunt as Fizzlepop Berrytwist
- Michael Peña as Grubber
- Taye Diggs as Capper
- Zoe Saldana as Captain Celaeno
- Kristen Chenoweth as Princess Skystar, a crown princess.
- Uzo Aduba as Queen Novo, Princess Skystar's Mother.
- Margot Robbie as Pyra Dagger, Fizzlepop's long lost sister
- Hayden Christensen as Buck Split, a earth pony and Vanhoover spy
- Ryan Reynolds as Lumpy Shifter, a pegasus and young Vanhoover desk agent, new to field work
- Elton John as Sonycwolv, a famous earth pony sensation
- Vin Diesel as Krozowlv, a pegasus
- Tyrese Gibson as Rhydon, a short-tempered rhino who's tough as a diamond
- Benedict Cumberbatch as Ironstar the Majestic, Starswirl's brother
- Nick Jonas as Mitchka, a earth pony and band (Eronica and Bustin's Brothers)
- Joe Jonas as Eronica, a unicorn and band (Mitchka and Bustin's Brothers)
- Kevin Jonas as Bustin, a pegasus and band (Mitchka and Eronica's Brothers)
- Eddie Murphy as Donkyle, a smooth donkey with a whacky voice
- Jamie Chung as Iguanita, a swift iguana with a serious ninja skills
- Vincent D'Onofrio as Scorchar, a burnt-eyed gibbon who hates ugly clothing
- Mark Ruffalo as Gammarax Stonebomb, Princess Celestia's lost leutenant
- Jack Black as Kuzcar, a young royal unicorn with a serious attitude
- Neil Patrick Harris as The Fire Lord, a new villain with a hot temper to match
- Ewan McGregor as Gorzan, a british gorilla who's a professional martial artist
- Owen Wilson as Ferrex, a western ferret who acts like a cowfolk
- Paul Bettany as Farluthor, a fashioned fox who's surprisingly a fashion kingpin
- John Goodman as Vultaur, a mohawk headed vulture who's an expert at history
- Liam Neeson as Fyrestorm Hammeraxe, Fizzlepop's long lost father
- Karen Allen as Cooler Freeze, Fizzlepop's long lost mother
- Tom Cruise as Frye Nolen, Fizzlepop's long lost uncle
- Arnold Schwarzenegger as Seahawk Patriot, Princess Celestia's lost general
- Charlize Theron as Nebula Cyclone AKA The Storm Queen, a 2nd new shocking villain
- Austin St. John as Jason
- Rebecca Shoichet as Sunset Shimmer
On February 15, 2019, Hasbro announced another feature length MLP movie is coming up with a scheduled release year of 2021. The Movie was referred as "My Little Pony 2"
- Universal Pictures Replaces Lionsgate as the distributor.