This article is rated G, meaning it is appropriate for all ages.
- David Berrn as Wilbur, a pig who lives on Homer's farm.
- E. G. Daily as Cardigan, a young black lamb who returned from the Second film.
- Amanda Bynes as Nellie, one of Charlotte's three daughters. She serves as their leader.
- Anndi McAfee as Joy, the cynical, sarcastic member of the trio.
- Maria Bamford as Aranea, the kinder, gentler member of the trio.
- Tom Kenny as Jeffrey, a young gosling who returned from the First film. He is Wilbur's old friend now he is friends with both Wilbur and Cardigan and he is the son of Gwen.
- Jim Cummings as Mr. Zuckerman, the current owner of Wilbur and Cardigan.
- Dawnn Lewis as Bessie, a brown female cow owned by Mr. Hirsch who returned from the Second film. She is friends with Wilbur and Cardigan.
- Charlie Adler as Templeton, a care-free, egotistical rat who lives at Homer's farm.
- Laraine Newman as Gwen, she is the goose who Wilbur befriended in the First film. She is Wilbur's friend and Jeffrey's mother.
- Debi Derryberry as Fern Arable, the spirited young girl who saved Wilbur in the First film.
- Rob Paulsen as Farley, an evil, pompous fox who returned from the Second film.
- Frank Welker as Animals' vocal effects
PARAMOUNT HOME ENTERTAINMENT and UNIVERSAL 1440 ENTERTAINMENT Presents
In Association with WALDEN MEDIA
A NICKELODEON MOVIES / THE KERNER ENTERTAINMENT COMPANY / NELVANA / UNIVERSAL ANIMATION STUDIOS Production
CHARLOTTE'S WEB 3: WILBUR AND CARDIGAN'S NEXT ADVENTURE